Produção Científica e Difusão do Conhecimento

A consolidação da pesquisa científica no BioMicro Lab reflete-se no impacto de suas publicações e na constante difusão de conhecimento inovador. Este repositório digital reúne a produção intelectual do nosso corpo docente, pesquisadores e alunos de pós-graduação, integrando estudos de ponta em microbiologia oral, dinâmica de biofilmes e o desenvolvimento de biomateriais. Acreditamos que a ciência de excelência deve ser acessível e transparente. Nesta seção, você encontrará artigos científicos publicados em periódicos internacionais de alto impacto, capítulos de livros, teses e dissertações que fundamentam as atividades do BioMicro Lab. Explore nosso acervo para consultar evidências científicas atualizadas, metodologias validadas e os desdobramentos epidemiológicos que conectam o trabalho de bancada diretamente com as estratégias de saúde coletiva.

Biblioteca Digital

Abaixo, encontra-se o catálogo cronológico de nossa produção científica de bancada e epidemiológica. Cada registro apresenta uma síntese dos objetivos e resultados alcançados, o corpo de pesquisadores envolvidos e as credenciais de indexação. Utilize os links de direcionamento para consultar o manuscrito integral ou acessar a publicação original via DOI (Digital Object Identifier).

Changes in taste perception in elderly population and its potential impact on oral health: a systematic review with meta-analysis Authors: Larisse Santos Mendonça Alves, Júlia Maria de Sousa Munduri, Mariana Caldas de Oliveira Mattos, Cristine Miron Stefani, Naile Dame-Teixeira Publication Date: December 05, 2024

Authors: Larisse Santos Mendonça Alves, Júlia Maria de Sousa Munduri, Mariana Caldas de Oliveira Mattos, Cristine Miron Stefani, Naile Dame-Teixeira.

Publication Date: December 05, 2024 Journal & Location: Frontiers in Oral Health | Department of Dentistry, School of Health Sciences, University of Brasília (UnB), Brasília, Brazil.

Brief Abstract: This rigorous systematic review and meta-analysis investigated whether older adults experience significant alterations in taste perception compared to younger adults. Analyzing 18 observational studies (including 2,728 individuals), the research demonstrated that elderly individuals require significantly higher concentrations to distinguish sweet, salty, and umami flavors. Interestingly, sour and bitter tastes did not show statistical differences between the age groups. From a clinical standpoint, this decline in taste acuity can lead older individuals to find high concentrations of sugars and salts more pleasant, potentially driving dietary changes. Increased sugar consumption can alter the oral microbiome, exacerbating periodontal diseases and root caries , highlighting the critical role of dental teams in monitoring taste disorders to promote healthy aging. Saiba mais….

Authors: Cecília de Brito Barbosa, Isabela Monici Silva, Naile Dame-Teixeira. 

Publication Date: April 04, 2024. Journal & Location: Journal of Applied Oral Science (JAOS) | Department of Dentistry, School of Health Sciences, University of Brasília (UnB), Brasília, Brazil.

Brief Abstract: This comprehensive state-of-the-art review explores the critical factors involved in the etiopathogenesis of root caries, focusing on how microbial collagenases breakdown the organic dentinal matrix. While conventional caries protocols focus primarily on biofilm-driven demineralization, recent molecular advancements reveal that proteolytic and periodontitis-associated bacteria (such as Porphyromonas) enrich subgingival lesions, overexpressing genes that encode bacterial collagenases—specifically from the U32 peptidase family. The study highlights that while the first stage of mineral loss can be reversed, the subsequent degradation of the exposed type I collagen matrix is irreversible. Proving the definitive clinical activity of these microbial enzymes remains a significant challenge. However, the review points to promising, low-cost translational strategies for root caries management, such as using natural cross-linking agents (like proanthocyanidins from grape seed extract) and anti-collagenolytic therapies to stabilize the root structure and prevent structural collapse.  Saiba Mais….

Authors: Jéssica Alves de Cena, Aline Belmok, Cynthia Maria Kyaw, Naile Dame-Teixeira.

Publication Date: February 23, 2024.  Journal & Location: Archives of Oral Biology | Department of Dentistry, School of Health Sciences, University of Brasília (UnB), Brasília, Brazil.

Brief Abstract: This comprehensive review and innovative in silico analysis maps the presence, methodology, and overlooked clinical significance of the human oral archaeome. While the bacteriome dominates dental research , low-abundance archaeal signatures—predominantly methanogens—are consistently present in subgingival plaques, infected root canals, and carious biofilms, showing strong correlations with severe periodontitis and painful endodontic infections. The study explores their syntrophic cross-feeding partnerships with proteolytic bacteria, suggesting they act as key components in oral dysbiosis. A critical highlight of the research is a rigorous in silico primer coverage analysis testing 44 primer pairs from existing literature against current databases. The authors revealed that several standard primers used in oral research possess 0% overall domain coverage, significantly underestimating real archaeal diversity. The study underscores an urgent methodological need to design updated, precise primer pairs targeting non-methanogenic groups (such as Nanoarchaeota and Thaumarchaeota) to accurately unlock the dark matter of the oral microbiome.Saiba mais….

Authors: Naile Dame-Teixeira, Reem El-Gendy, Andressa Souza de Oliveira, Cleonice Andrade Holanda, Luiz Antonio Soares Romeiro, Thuy Do.

Publication Date: August 02, 2024 Journal & Location: Microbiome | Department of Dentistry, School of Health Sciences, University of Brasília (UnB), Brasília, Brazil / School of Dentistry, University of Leeds, Leeds, UK.

Brief Abstract:This high-impact study successfully engineered and optimized a robust, clinically relevant in vitro dysbiotic biofilm model to mimic root caries development and test innovative microbial modulation strategies. Using a multi-volunteer saliva inoculum exposed to cariogenic sucrose cycles, the research evaluated two distinct pipelines: a “pre-treatment strategy” on bovine root slabs and a “post-treatment strategy” using a modified Calgary biofilm device coated with collagen and hydroxyapatite to screen targeted natural compounds. Through a comprehensive multi-methodological approach—including metatranscriptomic sequencing, confocal microscopy, scanning electron microscopy (SEM), and micro-computed tomography —the authors demonstrated significant shifts in the biofilm’s metabolic profile. The tested natural interventions effectively modulated key cariogenic pathways and suppressed microbial collagenase activity, halting mineral loss and safeguarding the organic dentinal matrix. This validated model establishes a powerful translational platform for evaluating non-invasive, targeted biomaterials to control root caries without disrupting oral cavity equilibrium. Saiba Mais…

Authors: Isabela Monici Silva, Cecília de Brito Barbosa, Jéssica Alves de Cena, Erick Ribeiro, Fernanda Cristina Pimentel Garcia, Cristine Miron Stefani, Naile Dame-Teixeira.

Publication Date: November 2024 Journal & Location: European Journal of Oral Sciences. Department of Dentistry, School of Health Sciences, University of Brasília (UnB), Brasília, Brazil.

Brief Abstract: This highly sophisticated systematic review and network meta-analysis evaluated the comparative in vitro efficacy of various synthetic and natural cross-linking agents used to stabilize dentin collagen against root caries progression. The research meticulously screened existing literature to determine which biomodification agents perform best in reducing both root caries lesion depth and the release of hydroxyproline—a definitive amino acid marker of collagen matrix breakdown. By executing a comprehensive network meta-analysis of laboratory data, the study establishes a clear statistical hierarchy among the tested agents (such as proanthocyanidins, glutaraldehyde, and carbodiimide). The mathematical model provides dental researchers with a robust, evidence-based guide for selecting the most potent therapeutic compounds to cross-link exposed type I collagen fibers, effectively halting organic matrix collapse and improving the long-term management of subgingival root lesions. Saiba Mais…

Authors: Larisse Santos Mendonça Alves, Júlia Maria Munduri, Israel Lacerda, Letícia Gonçalves Reis, Joana Rodrigues da Silva, Cristine Miron Stefani, Maria do Carmo Machado Guimarães, Luana Severo Alves, Solange Baraldi, Naile Dame-Teixeira

Publication Date: 2025 Journal & Location: Archives of Oral Biology | Department of Dentistry and Department of Nursing, School of Health Sciences, University of Brasília (UnB), Brasília, Brazil

Brief Abstract: This robust cross-sectional study evaluated 170 adult and elderly individuals (with and without Type 2 Diabetes Mellitus) to map how chronic oral conditions correlate with inadequate macronutrient intake and systemic health risks. The clinical data revealed that compromised oral parameters—such as significant tooth loss and the use of unstable dental prostheses—strongly impact dietary choices, shifting consumption away from essential fibers and proteins toward easily masticated, lower-quality carbohydrates and fats. Structurally, these dietary changes showed definitive associations with increased waist circumference risk, compromised weight status, and aggravated glycemic profiles in diabetic patients. The study underscores the critical need for interdisciplinary protocols connecting dental teams and nutritionists, demonstrating that rehabilitation of the oral cavity is a vital systemic countermeasure to prevent nutritional decline and manage metabolic disorders in aging populations. Saiba mais…

Authors: Camila Pinho e Souza Coelho, Joana Rodrigues da Silva, Rafaella Cristhina Rego Marques, Jéssica Luiza Mendonça Albuquerque de Melo, Larisse Mendonça Alves, Carlos Daniel Correa de Oliveira Melo, Eduarda Carvalho Leite, Priscilla Fernandes, Rafael Lara Brasil, Pedro Chagas Pereira Neto, Fernanda de Paula e Silva Nunes, Solange Baraldi, Debora Heller, Maria do Carmo Machado Guimarães, Érica Negrini Lia, Naile Dame-Teixeira.

Publication Date: 2025 Journal & Location: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology | School of Health Sciences, University of Brasília (UnB), Brasília, Brazil

Brief Abstract: This highly significant cross-sectional study investigated the definitive relationship between aging and salivary gland dynamics, establishing a precise annual rate of decline for both resting salivary flow (RSF) and stimulated salivary flow (SSF) while adjusting for critical confounding factors such as type 2 diabetes (T2D), polypharmacy, and dietary fiber intake. Analyzing clinical sialometry data from 166 individuals, the research revealed a striking prevalence of hyposalivation within the cohort. Through unadjusted and adjusted robust linear regression models, the study mathematically demonstrated that salivary output diminishes linearly with each advancing year of age. This reduction is heavily exacerbated by systemic chronic diseases and the cumulative burden of xerostogenic medications. The findings deliver a vital epidemiological tool for dental and medical teams, providing an objective baseline to monitor salivary dysfunction, prevent opportunistic oral infections, and develop targeted therapeutic strategies to preserve oral health and quality of life in aging populations. Saiba Mais…

Authors: J. R. da Silva, R. C. R. Marques, F. P. S. Nunes, A. A. Lima, D. Heller, C. M. Stefani, Naile Dame-Teixeira

Publication Date: 2025 Journal & Location: JDR Clinical & Translational Research (ou periódico correspondente de indexação) | School of Health Sciences, University of Brasília (UnB), Brasília, Brazil

Brief Abstract: This highly critical systematic review and meta-analysis re-evaluates and updates the decades-old baseline reference values used to define healthy salivary flow rates in contemporary adult populations. By pooling quantitative sialometry data from multiple global studies through a restricted maximum likelihood random-effects model, the research establishes definitive, updated normative averages for both unstimulated salivary flow (USF) and stimulated salivary flow (SSF) in healthy individuals aged 18 to 60. Furthermore, the study performs precise pairwise meta-analyses directly comparing healthy cohorts against patients suffering from hyposalivation-associated conditions (HACs). The resulting mathematical estimates provide an updated, evidence-based gold standard for clinical diagnostics and translational dental research, allowing teams to accurately identify early secretory dysfunctions and tailor precise therapeutic protocols. Saiba Mais…

Authors: Naile Dame-Teixeira, J. Lynch, X. Yu, Jéssica Alves de Cena, Thuy Do.

Publication Date: 2025 Journal & Location: Journal of Dental Research (JDR) | Department of Dentistry, School of Health Sciences, University of Brasília (UnB), Brasília, Brazil / School of Dentistry, University of Leeds, Leeds, UK

Brief Abstract: This high-level bioinformatic study mapped and compared the prevalence, diversity, and activity of the oral archaeome in carious and caries-free environments. Recognizing the traditional challenges in cultivating and isolating archaea, the research utilized a tailored shotgun metagenomic and metatranscriptomic analysis pipeline to screen large, publicly available datasets of human saliva and dental plaque. Reads were mapped against a custom-curated archaeal genome database using highly stringent filtering criteria (>97% similarity and 90% query coverage). The computational analysis revealed distinct patterns in archaeal prevalence and gene expression profiles between the cohorts. While certain methanogenic species showed a clear correlation with advanced carious lesions—likely driven by metabolic syntrophy with acidogenic bacteria—the study also identified specific archaeal signatures in healthy, caries-free samples. These findings significantly advance our understanding of non-bacterial dark matter in oral dysbiosis, highlighting archaea as active metabolic components rather than mere bystanders in dental caries pathogenesis. Saiba Mais…

Authors: Rafaella Cristhina Rego Marques, Joana Rodrigues da Silva, Larisse Santos Mendonça Alves, Bruna Leis Endres, Pedro Chagas Pereira-Neto, Letícia Gonçalves Reis, Carlos Daniel Correa de Oliveira Melo, Eduarda Carvalho Leite, Luana Severo Alves, Naile Dame-Teixeira

Publication Date: 2025 Journal & Location: Clinical Oral Investigations | Department of Dentistry, School of Health Sciences, University of Brasília (UnB), Brasília, Brazil

Brief Abstract: This comprehensive cross-sectional study decoded the intricate multi-parametric relationship between detailed salivary biochemical profiles and the real extent of both coronal and root caries lesions in adults, specifically evaluating how systemic hyperglycemic states modulate these oral dynamics. The clinical trial mapped structural and chemical parameters including salivary flow rate, pH, buffering capacity, viscoelasticity, and exact micro-concentrations of glucose, calcium, phosphate, and urea. Utilizing adjusted robust Poisson regression models, the research demonstrated that specific changes in salivary ion concentrations and physical properties significantly correlate with caries activity. Crucially, the data revealed that systemic hyperglycemia profoundly shifts these associations, altering the oral fluid’s protective equilibrium and accelerating organic dentinal matrix vulnerability. The findings deliver critical baseline data for targeted biomarker screening and non-invasive preventive materials calibrated for diabetic patient care. Saiba Mais…

Authors: Jéssica Luiza Mendonça Albuquerque de Melo, Fernanda de Paula e Silva Nunes, Camila Pinho e Souza Coelho, Debora Heller, Maria do Carmo Machado Guimarães, Thuy Do, Luiz Gustavo dos Anjos Borges, Daniela Corrêa Grisi, Naile Dame-Teixeira

Publication Date: 2025 Journal & Location: Lasers in Medical Science | Department of Dentistry, School of Health Sciences, University of Brasília (UnB), Brasília, Brazil

Brief Abstract: This innovative study presents a rigorous exploratory interim analysis of a randomized clinical trial investigating the therapeutic efficacy of low-level laser therapy (LLLT) and transcutaneous electrical nerve stimulation (TENS) targeting major salivary glands in type 2 diabetes mellitus (T2DM) patients suffering from salivary hypofunction. Moving beyond traditional sialometry assessments, the research pioneered the evaluation of how physical neurostimulation modalities actively modulate the composition, diversity, and dysbiotic equilibrium of the oral microbiome. Analyzing longitudinal resting and stimulated saliva samples, the clinical trial tracked immediate and post-intervention microbial shifts. The interim molecular and taxonomic profiling demonstrated that both LLLT and TENS therapies not only enhanced objective salivary secretion rates but also induced critical ecological changes, suppressing acidogenic pathways and promoting a healthier microbial balance. These findings establish a highly promising, non-invasive, and drug-free bio-hardware platform for treating xerostomia while preventing opportunistic opportunistic infections and controlling oral dysbiosis in metabolic disorders. Saiba Mais…

Editors: Naile Dame-Teixeira, Dongmei Deng, Thuy Do

Publication Year: 2025 Book Series: Advances in Experimental Medicine and Biology (Volume 1472) | Publisher: Springer Nature

Brief Abstract: This high-level, international scientific textbook provides a comprehensive, state-of-the-art compendium mapping the complex landscape of the oral microbiome and its critical bidirectional relationship with systemic health. Divided into specialized thematic chapters, the book explores the molecular mechanisms driving microbial symbiosis and the ecological shifts that precipitate oral dysbiosis—leading to prevalent conditions such as dental caries, periodontitis, and endodontic infections. Beyond diagnostic and fundamental microbiology, this volume delivers cutting-edge insights into advanced microbiome interventions, translational research models, and therapeutic strategies designed to restore oral equilibrium. Serving as an essential reference for postgraduate researchers, clinicians, and epidemiologists, this textbook bridges basic laboratory bench science with global healthcare strategies, underscoring the oral cavity as a vital mirror and modulator of general human well-being. Saiba Mais….

Authors: Naile Dame-Teixeira, Jéssica Luiza Mendonça Albuquerque de Melo, Clarissa Cavalcanti Fatturi Parolo

Publication Date: January 15, 2026 Journal & Location: Caries Research | Department of Dentistry, School of Health Sciences, University of Brasília (UnB), Brasília, Brazil / Department of Preventive and Social Dentistry, School of Dentistry, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, Brazil

Brief Abstract: This forward-thinking review article challenges conventional, reductionist dental microbiology by advocating a profound paradigm shift in managing dental caries: moving from pathogen eradication (“blame”) to ecological equilibrium (“balance”). Capitalizing on recent multi-omics, next-generation sequencing, and oral archaeome discoveries, the authors demonstrate that functional diversity and metabolic activity within the biofilm operate dynamically at the individual level, far beyond isolated taxonomies. The study connects ecological resilience theory with molecular data, establishing three central pillars to guide future translational research. By framing dysbiosis as a modifiable community-wide metabolic shift rather than an infection by specific bacteria, this framework delivers a powerful, evidence-based roadmap for designing targeted biomaterials and non-invasive, eco-friendly therapies aimed at stabilizing the oral microbiome without disrupting host homeostatic health. Saiba Mais…

Coordenadora: Profª. Drª. Nailê Dame Teixiera - Sala BT 138/13 - Faculdade de Ciências da Saúde - Universidade de Brasília - Campus Universitário Darcy Ribeiro, s/n Asa Norte CEP:70910-900  Brasília - DF Brasil - E-mail: biomicrolab@unb.br

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